HIGH CONTENT IMAGING ASSAYS
The inhalation safety testing of new medicines and products often includes the analysis of alveolar macrophage responses. ImmuONE’s in vitro alveolar macrophage-like models can be used to generate reproducible quantitative data about macrophage responses to inhaled substances. Our research has shown that or high content image analysis (HCIA) of macrophages in monoculture can be used to differentiate between safe and unsafe immune responses to substances.
Many substances are deemed dangerous to humans after being shown to induce abnormal alveolar macrophage morphology, whereby the cell takes on a ‘foamy’ appearance. However, relatively little is known about the ‘foamy’ macrophage response and whether it truly is harmful to the lungs. ImmuONE examines the mechanisms and characteristics of the response and can directly correlate the results to in vivo equivalent studies. ImmuONE has cultivated specific expertise in this domain and our experts can guide you smoothly through the analysis of these results.
Through individual cell analysis and layering of cellular features, we can generate detailed phenotypic profiles to illustrate how macrophages respond to the compounds of interest. We have created a database of reference compounds with established in vivo responses, to which we can compare any result. By comparing the phenotypic profiles of new compounds to those in our reference database, we can predict whether these new compounds are safe.
Images of ImmuPHAGE™ before (left) and after (right) amiodarone exposure. ImmuPHAGE™, our human alveolar macrophage-like model, can be used in morph_ONE assays to provide mechanistic understanding and categorisation of macrophage response.