ImmuLUNG™

Introducing ImmuLUNG™

The ImmuLUNG™ cell model developed by ImmuONE can help predict how lung epithelial and immune cells respond to inhaled substances. Used in-house as part of ImmuONE’s services, ImmuLUNG™ combines human alveolar epithelial cells and alveolar macrophage-like cells in a 3D environment.

Human alveolar-like macrophages

Human alveolar epithelial cells​

WHAT IS IMMULUNG™?

ImmuLUNG™ combines human alveolar epithelial cells and alveolar macrophage-like cells in a 3D environment. The interactions between these two cell types provide better physiological representation, enabling a more accurate and in-depth understanding of how inhaled products interact with the airways in the deep lung.

Key Features and Benefits:

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How it works

The model is composed of alveolar macrophage-like cells assembled in vitro in well plates, that closely represent the form and function of human cells. Substances are added to the well plates, and adverse responses can then be evaluated. ImmuPHAGE™ is fully validated and characterised for cell structure & viability, phagocytic activity and cytokine response. ImmuPHAGE™ is available for purchase as a product and as part of ImmuONE’s service packages.

Watch our video below to see how to use ImmuPHAGE™

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How it works

ImmuLUNG™ combines human alveolar epithelial cells and alveolar macrophage-like cells in a 3D environment. The resulting cellular crosstalk can better recreate lung tissue functions and immune response modulations.

Available as an in-house service at ImmuONE, these immortalised human alveolar epithelial and macrophage-like cells are preassembled on tissue culture well plates.  A range of test materials can be applied to the model in solution, suspension or as an aerosol, and the biological responses of individual cells or the whole system can be monitored.

ImmuLUNG™ is currently available as part of ImmuONE’s service packages – coming soon as a standalone product.

USES FOR IMMULUNG™

Inhalation toxicology

Inhalation toxicology

to understand the safety and fate of inhaled substances

Cytotoxicity

Cytotoxicity

to assess substance effects on membrane integrity and viability

Cell stress

Cell stress

to monitor cell’s mitochondrial activity and how they handle damaging reactive oxygen species

Morphometric assessment

Morphometric assessment

to evaluate morphological changes within a cell and population, used with high-content analysis

Safety pharmacology

Safety pharmacology

to measure phagocytosis and macrophage polarisation

Inflammation

Inflammation

to identify pro-inflammatory cytokines and chemokines critical for regulating inflammation in the alveolus

Epithelial barrier integrity

Epithelial barrier integrity

evaluating and monitoring tight junctions between cells through paracellular permeability assays, and epithelial tissue through transcatheter edge-to-edge repair (TEER) assays

Epithelial permeability assessment

Epithelial permeability assessment

flux, P app , cellular uptake

Advantages of using ImmuLUNG™

Available formats for ImmuLUNG™

By default, ImmuLUNG is available in an air-liquid interface 24-well format for our in-house services. Other formats are available on request. 

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