ImmuPHAGE™

Introducing ImmuPHAGE™

ImmuPHAGE™ is an in vitro cell model of human alveolar macrophages that facilitates the investigation of complex interactions occurring between inhaled compounds and immune cells in the deep lung. ImmuPHAGE™ can evaluate the impact of a wide range of substances on the human lungs

Functional characteristics

Phenotypic Characteristics

WHAT IS IMMUPhage™?

ImmuPHAGE™ features human alveolar macrophage-like cells that closely mirror the physiological and functional attributes of native tissue-resident cells. This model can provide a detailed understanding of the immune processes found in the small airways, including particle clearance, inflammation response, and changes in cell function (e.g. foamy macrophages). 

More than a tool for just assessing cell viability, ImmuPHAGE™ provides a profound insight into the impact of chemicals and particles on human alveoli. This patented technology serves as an invaluable asset to diverse sectors, including cosmetics, tobacco, fragrances, agri-chemicals, pharmaceuticals, and academic research. Its ethical and streamlined approach can, in certain scenarios, entirely replace animal testing or significantly reduce the requisite number of animals. ImmuPHAGE™ marks a revolutionary stride in promoting humane research methodologies

Key Features and Benefits:

(*when cultured in-house in ImmuONE laboratories as part of our services)

ImmuPHAGE™ is accessible as part of ImmuONE’s service packages and can also be procured in plate or vial format.

ImmuONE specialises in human alveolar
macrophage responses and epithelial cell culture
platforms

Why is it
important?

Alveolar macrophages are often the first cells to respond to an inhaled substance in the deep lung. Their reactions are key determinants of how inhaled substances affect the lungs.

At ImmuONE, we strive to provide relevant immunological and biochemical readouts that can be linked to mechanisms of toxicity. Most alternative cell platforms only indicate cell viability, but ImmuPHAGE™ delves much deeper, offering a unique insight into the impact of chemicals and particles on human alveoli.

In addition, ImmuONE’s scientists are highly specialised in lung alveolar interactions and can provide expert analysis of the results elicited by ImmuPHAGE™. We can benchmark results for a new compound against our reference database to put test results in context, helping our clients answer safety questions with confidence.

This versatile immune model can be applied to problems faced by a range of industries i.e., chemicals, consumer products, pharmaceuticals and many others.

ImmuPHAGE™ is optimised to assess the safety risks of different products, closely mimicking the effects observed in human lungs. It highlights lower airway immune processes, i.e., particle clearance, inflammation, cell functionality and morphological changes (i.e. foamy macrophages) promoting the advancement of ethical and streamlined evaluation techniques.

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How it works

The model is composed of alveolar macrophage-like cells assembled in vitro in well plates, that closely represent the form and function of human cells. Substances are added to the well plates, and adverse responses can then be evaluated. ImmuPHAGE™ is fully validated and characterised for cell structure & viability, phagocytic activity and cytokine response. ImmuPHAGE™ is available for purchase as a product and as part of ImmuONE’s service packages.

Watch our video below to see how to use ImmuPHAGE™

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product
insights

Uses for ImmuPHAGE™

Inhalation studies

Inhalation studies

to understand the safety and fate of inhaled substances

Cytotoxicity

Cytotoxicity

to assess substance effects on membrane integrity and viability

Cell health and functionality

Cell health and functionality

compatible with a wide range of standard cell health and metabolic assessments to ascertain acute toxicity

Morphometric assessment

Morphometric assessment

to evaluate morphological changes within a cell linking to morphological changes observed in vivo and to support mechanistic understanding of cellular changes

Macrophage polarisation

Macrophage polarisation

assessment of cells to proinflammatory (M1) and anti- inflammatory (M2) activation status

Inflammation response assessments

Inflammation response assessments

to identify pro-inflammatory cytokines, chemokines and cell surface markers critical for regulating inflammation in the alveolus

Inhalation studies

Inhalation studies

to understand the safety and fate of inhaled substances

Cytotoxicity

Cytotoxicity

cytotoxicity

Cell health and functionality

Cell health and functionality

compatible with a wide range of standard cell health and metabolic assessments to ascertain acute toxicity

Morphometric assessment

Morphometric assessment

to evaluate morphological changes within a cell linking to morphological changes observed in vivo and to support mechanistic understanding of cellular changes

Macrophage polarisation

Macrophage polarisation

assessment of cells to proinflammatory (M1) and anti- inflammatory (M2) activation status

Inflammation response assessments

Inflammation response assessments

to identify pro-inflammatory cytokines, chemokines and cell surface markers critical for regulating inflammation in the alveolus

Benefits of using ImmuPHAGE™

Available formats for ImmuPHAGE™

To allow flexible dosing ImmuPHAGE™ is available as

96- and 24-well plate

96- well imaging plates

In a vial of ready-to-use-suspended cells

Fill out the form below to receive your datasheet

For orders or quotations

Email us at info@immuone.com

Find out more

That’s a wrap for 2023!

New innovations, new research, new teammates, a new lab – and soon, a new year! Before we turn our attention to 2024, let’s take a step back and appreciate all we’ve achieved so far.

Highlights from ImmuONE’s  First Webinar  

ImmuONE made a significant stride on November 8th by hosting its first-ever webinar, ‘unveiling insights into the next generation of tools for inhalation risk assessment’. The webinar began with a glimpse into ImmuONE’s evolution, emphasizing key milestones and leadership perspectives, presented by Prof. Victoria Hutter.

For orders or quotations

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