Therapeutic areas
ImmuONE focuses on respiratory and immunological in vitro assays and can carry out a wide range of different cell culture assays in this area. We are also expanding into other organs and diseases where tissue-resident macrophage responses can provide further insight into human safety including lung diseases, the eye and atherosclerosis. We can design tailor-made assessments to meet your testing needs in the below areas.
Respiratory
At ImmuONE, our expertise spans the entire respiratory system, from the mouth and nose, right down to the alveolus. We utilise a diverse range of in vitro cell culture models, including proprietary, non-proprietary, and those developed in-house, ensuring the utmost human physiological relevance for safety assessments. Whatever your requirements, we are committed to offering the most suitable models to meet your needs.
Alveolar
Establishing the safety of new products for inhalation often requires an assessment of how the substance interacts with both physical and immunological barriers in the alveolus. ImmuONE’s scientists have years of in-depth expertise in assessing the responses of alveolar epithelial and immune cells.
In addition to all standard commercially available cell models and assays, we use our patented ImmuLUNG™ and ImmuPHAGE™ models to carry out an extensive range of cell culture assays relevant to the alveolar region as the needs of the client dictate.
In addition to all standard commercially available cell models and assays, we use our patented ImmuLUNG™ and ImmuPHAGE™ models to carry out an extensive range of cell culture assays relevant to the alveolar region as the needs of the client dictate.
Nasal, bronchi and bronchiolar regions
We work with epithelial models of both the conducting and respiratory airways, including a variety of commercially available human primary tissue models, as well as human and animal cell line models as described in scientific literature. We conduct both standard and bespoke assessments with these models to fit your scientific inhalation requirements. We can incorporate macrophage or monocyte immune cells into conducting airways models to enhance the understanding of immune and inflammatory response in these tissues.
Inflammation
Inhalation toxicology has traditionally leaned on epithelial cell models for alveolar safety assessment. While these models provide insights into barrier function and cytotoxicity, they may not comprehensively address the multifaceted inflammatory responses integral to long-term lung health, especially in the context of chronic exposure.
The sentinel role of alveolar macrophages, the lung’s immediate responders, cannot be overstated. They are not just indicative of immediate reactions but also offer a window into the broader health ramifications within the respiratory system. At ImmuONE, we recognise the significance of this complex interplay. Hence, we champion the use of immune-competent models, focusing on the intricate dynamics of alveolar macrophages. By doing so, we endeavour to offer a more holistic understanding of the inflammatory impact and overall safety of inhaled substances.
We typically offer clients a wide range of in vitro alveolar macrophage assays that can assess cytokine expression, macrophage polarisation, phagocytic activity and more. With our experts, you can study inflammatory and immunological responses in the airways. We can also help you design novel experiments and made-for-you assays. Find out more about our inhalation services here.
Inflammatory diseases of the airways
Alveolar macrophages are fundamental in airway inflammation and disease. We are constantly innovating in this area and are currently working on acute and chronic inflamed airways models as well as idiopathic pulmonary fibrosis.
Contact us if you would like further information on this area or if you would like to work with us.
Contact us if you would like further information on this area or if you would like to work with us.
Atherosclerosis
Macrophages play a central role in the development of plaques found in atherosclerotic cardiovascular disease. Classically activated pro-inflammatory macrophages are implicated in initiating and sustaining plaques while alternatively activated anti-inflammatory macrophages are linked to the resolution of inflammation and plaque stabilisation.
We are currently developing lipid_ONE, an assay designed to predict how the intracellular lipid profiles of macrophages alter with compound exposure. lipid_ONE could be used to predict whether exposure to a substance contributes to the initial events that lead to atherosclerosis.
Please enquire to learn more about how our work in this area could support your needs.
ImmuONE embraces challenges and we are always looking to apply our immune expertise to new organs and areas of the body. If you’re interested in working with us on something new, please get in touch!
Email us today to find out more about our services or discuss potential collaborations.