In our recent ImmuBYTES webinar, we delved into the fascinating world of Cell Painting, a high-content imaging assay (HCIA) that transforms cellular morphology into a rich source of data. 

Key takeaways from the webinar:

• Cellular Detail: We explored how cell painting utilises fluorescent dyes to label different cellular components such as the nucleus, mitochondria, and cytoskeleton, generating vibrant images that reveal intricate cellular details.
• Focus on Alveolar Macrophages: We highlighted how ImmuONE leverages cell painting to study alveolar macrophages, immune cells critical for lung health. By analysing these cells, we can gain insights into how various substances, from inhaled particles to potential therapeutics, impact their function.
• Single-Cell Precision: We emphasised the power of single-cell analysis, where individual cells are assessed, enabling us to understand how different substances affect diverse cell populations within a culture.
• Key Morphological Descriptors: We discussed key morphological descriptors, such as mitochondrial activity, cell area, and vacuolation (number and size), which provide critical clues about potential adverse effects.
• Reference Library for Healthy Macrophages: ImmuONE has meticulously built a reference library of compounds to establish a baseline for “healthy” macrophage morphology. This library serves as a valuable resource for identifying deviations from normal cell behaviour, indicating potential toxicity.

Watch the full webinar video below to gain a better understanding and visualisation of the scientific data or read this blog on the Scientist.com: