INNOVATIVE SOLUTIONS
FOR INHALATION
SAFETY

In vitro specialists for
immune responses in the lung

DELIVERING IN VITRO SOLUTIONS

In vitro services for inhalation testing

In vitro alveolar macrophage assays

Immune-competent in vitro human airways models

ImmuONE delivers innovative respiratory in vitro cell culture products and services focusing on innate immune responses

Generating Human-relevant results with multiple endpoints

Standard portfolio

Our contract research services with leading experts provide a tailored experimental design to directly address your in vitro inhalation assessment needs.

Advanced multi-metric assessments

We use state-of-the-art high content imaging techniques to combine multiple endpoints for immune cells which support mechanistic understanding and can directly translate to clinical studies.

Novel immune-competent products

Our patented products ImmuPHAGE™ and ImmuLUNG™ provide a detailed understanding of the immune response in the lungs to inhaled chemicals and products.

Generating human-relevant results with multiple endpoints

Standard portfolio

Our contract research services with leading experts provide a tailored experimental design to directly address your in vitro inhalation assessment needs

Advanced multi-metric assessments

We use state-of-the-art high content imaging techniques to combine multiple endpoints for immune cells which support mechanistic understanding and can directly translate to clinical studies

Novel immune-competent products

Our patented products ImmuPHAGE™ and ImmuLUNG™ provide a detailed understanding of the immune response in the lungs to inhaled chemicals and products

Importance of airway immune responses for inhaled products

Along with the air we inhale, each breath also contains a cocktail of chemicals and particles from our environment. The immune system in the lungs interacts with these chemicals and determines the body’s response to them. Despite respiratory immunology being a key aspect of inhaled product safety, in vitro models and assays to mimic these responses was an unmet need and animal experiments have traditionally been heavily relied upon to provide this information.

Since 2013, researchers at ImmuONE have been working to provides a solution to this problem.

We are experts in the lung’s immune system – in particular, the alveolar macrophages which are the first to respond when a chemical enters the respiratory system. Our services, products and experts enable you to understand, in detail how alveolar macrophages respond to inhaled chemicals/particulates and support classification of the response as being adaptive or adverse.

Our unique in vitro models and multi-endpoint assessments deliver results spanning morphology, function and biochemistry to provide companies with clarity on the human health impact of their products. We provide a a cost-effective, multi-endpoint alternative which is more human-relevant and reduces animal testing, exploring various interpretations of a read-out.

We carry out a wide variety of in vitro services in-house, with a specialisation in alveolar cell painting assays. Our morph_ONETM assay combines detailed cell morphology with cell health and biochemical readouts to help classify the mechanism of ‘foamy’, highly vacuolated macrophages to support safety assessment. Our two novel products – ImmuPHAGE™ and ImmuLUNG™ – provide in-depth analysis of macrophage responses in the lungs and epithelial tissue of the alveoli.

Importance of airway immune responses for inhaled products

Historically, alveolar safety has relied on epithelial cell models, which, though convenient, offer a narrow scope—primarily focusing on barrier function or cytotoxicity. Such limited endpoints may overlook potential long-term adverse effects on lung health, especially concerning inflammation from chronic product administration. The lung’s first responders, alveolar macrophages, play a pivotal role in homeostasis and regulating inflammation. Inflammation is not only a marker of acute reactions but also indicative of long-term health implications in the respiratory tract. Therefore, to ensure the comprehensive safety of inhaled products, it’s imperative to employ immune-competent models that capture the nuanced responses of alveolar macrophages. Only through understanding these responses can we truly gauge the safety and potential inflammatory impact of inhaled substances.

Our services, products and experts enable you to understand, in detail, how alveolar macrophages respond to inhaled chemicals/particulates and support classification of the response as being adaptive or adverse.

Our unique in vitro models and multi-endpoint assessments deliver results spanning morphology, function and biochemistry to provide companies with clarity on the human health impact of their products. We provide a cost-effective, multi-endpoint alternative which is more human-relevant and reduces animal testing, exploring various interpretations of a read-out.

We carry out a wide variety of in vitro services in-house, with a specialisation in alveolar high content imaging assays. Our morph_ONE™ assay combines detailed cell morphology with cell health and biochemical readouts to help classify the mechanism of ‘foamy’, highly vacuolated macrophages to support safety assessment. Our two novel products – ImmuPHAGE™ and ImmuLUNG™ – provide in-depth analysis of macrophage responses in the lungs and epithelial tissue of the alveoli.

IMPORTANCE OF AIRWAY IMMUNE RESPONSES

Understanding the inflammatory response of the airways to inhaled substances is paramount to assessing the long-term adverse effects on lung health. Many inhaled substances reach the small or distal airways, and it is in this region of the lung where alveolar macrophages are the first responders to these substances and play a pivotal role in homeostasis and regulating inflammation. Therefore, to ensure a comprehensive safety of inhaled products, it’s imperative to employ immune-competent models that capture the nuanced responses of alveolar macrophages.

At ImmuONE, we specialise in alveolar macrophage models and responses. Our services, products and experts enable you to understand, in detail how alveolar macrophages respond to inhaled chemicals/particulates and support classification of the response as being adaptive or adverse.

The advantages of in vitro

We tailor our approach to match your needs – whether that means carrying out in-depth, multi-endpoint analyses in-house for you, whether you need us to design a novel experiment to test your hypothesis, or whether you want to avail of our ImmuPHAGE™ technology in your own lab, with our support.
Testing on human cells gives you an accurate human response
Cuts the costs of lengthy preliminary animal trials
ImmuONE’s improved processes mean that multiple, specific endpoints are possible
Tight control of physical and chemical conditions mean easily reproducible and more robust results
Higher throughput