Following successful completion on the phase 1 challenge awarded to ImmuONE by the NC3Rs, we were announced as the winners for the phase 2 challenge in Jan 2022. Sponsored by Unilever and AstraZeneca the project aims to establish and develop in vitro OECD test guideline assays; free from animal-derived products and demonstrate their equivalence and superiority to current strategies. Once optimised, these technologies will be progress to a commercially ready, human relevant, animal-free bioassays with the view to gaining regulatory acceptance.