ImmuPHAGE™ represents functional alveolar macrophages in vitro.
It consists of human alveolar-like cells which closely resemble the features of alveolar macrophages found in the deep lung, such as the ability to elicit an immune mediated response to inflammatory modulators and alveolar clearance of particulate material.
The standardised model is fully validated and characterised in terms of morphology, viability, phagocytic activity and cytokine response. ImmuPHAGE™ can provide highly reproducible toxicological information and detailed mechanistic understanding of the safety and fate of inhaled substances in the lungs
Morphologically resembling human primary alveolar macrophages
Morphology of ImmuPHAGE™ (left) closely resemble primary human alveolar macrophages (right)
Non-proliferating cells with a long shelf life
ImmuPHAGE™ proliferation (left) and cytotoxicity (right) were evaluated over 35 days of culture. ImmuPHAGE system did not show proliferation in long term culture confirming terminally differentiation which is one of the features of human alveolar macrophages. ImmuPHAGE™ cells were viable for up to 5 weeks, showing cytotoxicity below 10% of the whole cell population